Accelerated Solvent Extraction of Phenols from Lyophilised Ground Grape Skins and Seeds

The efficient extraction of phenols from grapes is an important step for their reliable quantification. aim was to optimise the lyophilisation process and grape skins seeds. phenol yield lyophilised tissues investigated with different accelerated solvent (ASE) operating conditions. Skins seeds were separated frozen berries without being ground. weight loss during followed daily. Phenols extracted lyophilised, cryo-ground ASE at room temperature 10.3 MPa using 80% aqueous acetone 60% methanol. effects operational parameters (the number cycles (ECs) static time (ST) duration) investigated. evaluated spectrophotometrically by determining total phenolic index 280 nm (TPI). significantly dropped after 24 h continued decrease, although not significantly, up until 9th day. optimal estimated be 3 days 5 seeds, respectively. affected changes Based on TPI, conditions as follows: (i) seeds—eight ECs 10 min ST then four 20 methanol; (ii) skins—eight 1 one EC